Research Progress in the Use of Biosensors to Detect Fat Soluble Vitamins

NIE Aiai; QIU Manyan; ZHAO Qianyu; WANG Xu; MAN Chaoxin; JIANG Yujun; PEI Xiaoyan; GUO Ling

doi:10.13982/j.mfst.1673-9078.2025.12.1306

Abstract：Fat-soluble vitamins are essential micronutrients for human growth and development,with deficiencies in these vitamins posing a serious threat to human health.Furthermore,the majority of fat-soluble vitamins cannot be synthesized in the human body and,therefore,must be obtained from external sources.Therefore,rapid and sensitive detection of fat-soluble vitamins is crucial to ensure safe vitamin intake.Although traditional methods such as high-performance liquid chromatography,high performance liquid chromatography,tandem mass spectrometry,and supercritical fluid chromatography offer advantages in terms of selectivity and sensitivity,they also present disadvantages,including complex sample pre-treatment,time-consuming procedures,and dependence on large-scale instrumentation.These constraints have limited the applicability of traditional methods in the rapid detection of fat-soluble vitamins.Recently,biosensors have been used for the detection of fat-soluble vitamins owing to their advantages of high specificity,high sensitivity,low cost,suitability for on-site analysis,and ease of operation.This review initially outlines the physiological functions of common fat-soluble vitamins.Subsequently,it analyzes the advantages and disadvantages of traditional methods and biosensors in detecting fat-soluble vitamins.Finally,it provides a comprehensive summary of the latest research progress on biosensors in the detection of fat-soluble vitamins.This paper outlines the biological components,linear detection range,sensitivity,and detection limits of lipid-soluble vitamin detection biosensors,providing a foundation for the development of future lipid-soluble vitamin detection biosensors.