Rapid Determination of Trace Benzo[a]pyrene in Food Using A Magnetic Co3O4 Nano-electrochemical Sensor
YUAN Zhaohui
SONG Ziwei
HE Huange
LI Shuguo
Abstract：To establish a rapid and sensitive method for detecting trace amounts of benzo[a]pyrene(BaP)in food,an AB@Co3O4@MWCNTs-COOH nano-electrochemical sensor was developed using Acetylene Black(AB),magnetic cobalt oxide(Co3O4)nanoparticles,and carboxylated carbon nanotubes(MWCNTs-COOH).The composite material AB@Co3O4@MWCNTs-COOH was used to modify the electrode surface.The electrochemical reaction of BaP on various sensors was investigated using cyclic voltammetry(CV).A distinct oxidation peak with no corresponding reduction peak was observed,indicating an irreversible oxidation-reduction process.The AB@Co3O4@MWCNTs-COOH sensor was characterized by scanning electron microscopy(SEM),CV,and electrochemical impedance spectroscopy(EIS),and the operating conditions were systemically optimized.The optimal conditions were as follows:electrolyte solution of lithium perchlorate(0.3 mol/L,pH 2);a modification solution volume ratio of VCo3O4@MWCNTs-COOH to VAB was 2:1,a total modification volume of 6 μL,and an enrichment time of 15 min.Under these conditions,the oxidation peak current of BaP showed a strong linear correlation with concentration,with the regression equation Ip=3.1939CBaP+1.5439(R²=0.996).The limit of detection(LOD)was 3.12×10-10 mol/L.Application of this method to barbecued meat samples yielded recovery rates ranging from 84.34％to 93.36％.The proposed sensor demonstrates ease of operation,ultra-low LOD,strong anti-interference ability,and high stability,making it suitable for rapid on-site screening and quantitative analysis of BaP in food.
Keywords：benzo[a]pyrenefood safetynano-electrochemical sensorrapid detection
Publication Date：2025-11-20
Online Publishing Date：2025-12-26(First online date of this platform, not the publication date of the document)
Pages：10( 313-322 )