Comparison on the Physicochemical Characteristics and Inhibitory Activity against α-Glucosidase of the Polysaccharides with Different Molecular Weights from Phyllanthus emblica L.
FANG Jiaqin
ZHANG Tong
FU Shuyong
FU Xiong
GUO Weixiong
CHEN Chun
Abstract：To examine the relationship between structure and activity of Phyllanthus emblica L.polysaccharides,the polysaccharides were extracted using hot water then precipitated with 50%and 80%(V/V)ethanol into two fractions(PEP-50 and PEP-80).PEP-50 and PEP-80 had the same composition of monosaccharides,but their contents differed to some extent,with galacturonic acid being the main constituent.Besides,the molecular weight of PEP-80 was primarily 234.45 kDa(accounting for 99.21%),which was lower than that of PEP-50(primarily 256.26 kDa;accounting for 92.51%).Moreover,both PEP-50 and PEP-80 exhibited hypoglycemic activity,with their IC50 values being 3.34 and 0.46 mg/mL,respectively.Both acted as mixed-type inhibitors against α-glucosidase.Compared with PEP-50,PEP-80 exerted a stronger inhibitory effect against α-glucosidase,exhibiting stronger abilities to bind to free α-glucosidase and to α-glucosidase-substrate complex.These differences wereclosely associated with their different chemical compositions and molecular weights.The above experiments suggest that the molecular weight of Phyllanthus emblica L.polysaccharides had a high impact on their hypoglycemic activity,which provides a theoretical basis for future development of healthy foods using different types of Phyllanthus emblica L..
Keywords：Phyllanthus emblica L.polysaccharidesgradient ethanol precipitationphysicochemical characterizationhyperglycemic activity
Publication Date：2025-11-20
Online Publishing Date：2025-12-26(First online date of this platform, not the publication date of the document)
Pages：9( 144-152 )