Antiaging Effects of Acer truncatum Seed Oil-based Nanostructured Lipid Carriers on Caenorhabditis elegan

YAN Yanfang; LI Xiaohong; LIU Sha; RAN Xianting; YAN Ling; CAO Jiafu; YANG Juan

doi:10.13982/j.mfst.1673-9078.2025.9.0828

Abstract：The properties of Acer truncatum seed oil-based nanostructured lipid carriers(ASO-NLC)prepared using the melt emulsification-ultrasonication method were characterized,and their effects on the longevity,fertility,exercise capacity,antioxidant capacity,heat/oxidative stress,and apoptosis of Caenorhabditis elegans were evaluated.ASO-NLC exhibited a particle size of 244.40 nm,a polydispersity index of 0.17,a zeta potential of-30.07 mV,and good centrifugal stability,that it is a stable oral formulation.Comparisons between the treatment groups and the control group(NC)revealed that ASO-NLC could extend the longest lifespan by more than 50.21％and the average lifespan by 12％～36％.Moreover,the extension of life span did not reduce the fertility of C.elegans.ASO-NLC also significantly improved the motility of C.elegans after 10 days of culture,with more obvious enhancement observed on day 15.Additionally,ASO-NLC increased the activities of glutathione peroxidase,catalase,and superoxide dismutase by 21.58％～48.81％,22.42％～58.58％,and 16.11％～75.88％,respectively,while reducing the malondialdehyde content by 6.81％～34.89％.Under acute oxidative stress,the longest survival time of C.elegans in the ASO-NLC group was 1.50-fold longer than that in the NC group.The acridine orange staining results demonstrated that the relative fluorescence intensity of the ASO-NLC group was 13.71％～17.00％,which was significantly lower than that of the NC group(P＜0.01).In conclusion,ASO-NLC can increase the lifespan,exercise capacity,and stress resistance of C.elegans;enhance the in vivo activities of antioxidant enzymes;and reduce apoptosis,enabling ASO-NLC to slow the aging of C.elegans.This study provides a theoretical basis for the development of functional products of A.truncatum seed oil.