Protective Effect of Black Corn Polysaccharide on Renal Injury in Diabetic Mice
WU Yixing
WANG Yan
YE Zhiming
YANG Huiwen
LIU Bing
YOU Tianhui
Abstract：This study aimed to explore the protective effects and underlying mechanisms of black corn polysaccharide on renal injury in diabetic mice.A murine diabetes mellitus model was established using intraperitoneal streptozocin injection,followed by black corn polysaccharide intervention.Urine albumin content,urine albumin/creatinine ratio,serum creatinine(SCr),blood urea nitrogen(BUN),triglyceride(TG),total cholesterol(TC),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),catalase(CAT),and malondialdehyde(MDA)levels were measured using biochemical assay kits.Histopathological changes in kidney tissues were assessed through HE,PAS,and Masson staining.Enzyme-linked immunosorbent assay(ELISA)was employed to measure the levels of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-alpha(TNF-α),and interleukin-10(IL-10).Western blot analysis was performed to determine the protein expression of phosphorylated p65(p-p65),p65,nuclear factor erythroid 2-related factor 2(Nrf2),and heme oxygenase-1(HO-1).The results showed that compared with those in the model group,the levels of SCr,BUN,MDA,TG,and TC in the high-dose black corn polysaccharide group were reduced by 56.41%,54.89%,45.34%,13.10%,and 11.85%,respectively.Additionally,the 24 h urinary protein content and UACR levels decreased(P<0.01),while the activities of SOD,GSH-Px,and CAT increased(P<0.01).The renal tubules were neatly arranged,and the degree of fibrosis and glycogen deposition in the kidney tissue was reduced.The serum levels of pro-inflammatory factors IL-1β,IL-6,and TNF-α were reduced by 36.85%,30.77%,and 36.85%,respectively,while the level of the anti-inflammatory factor IL-10 was increased by 32.46%(P<0.01).The expression of Nrf2 and HO-1 was increased by 68.33%and 77.09%,respectively,while the expression of p-p65/p65 was decreased by 35.22%.These results indicate that black corn polysaccharide exerts a considerable protective effect on diabetic kidney damage,offering a scientific basis for the treatment of patients with clinical diabetic nephropathy.
Keywords：black corn polysaccharidestreptozocinhypoglycemicantioxidantdiabetic nephropathy
Publication Date：2025-08-20
Online Publishing Date：2025-10-10(First online date of this platform, not the publication date of the document)
Pages：9( 20-28 )