Protective Effect of Resveratrol on Oxidative Casein-induced Learning and Memory Impairments in Mice

MO Ling; LU Hangsun; FU Henghui; DENG Qiuling; ZHAO Chaochao; LI Jingjing

doi:10.13982/j.mfst.1673-9078.2025.8.0613

Abstract：To explore the protective effect of resveratrol on oxidative casein-induced learning impairments in mice and the possible underlying mechanism,thirty-six 7-week-old Kunming male mice were randomly divided into four groups:the blank control group(CT),oxidized casein group(CO),resveratrol low-dose group(LR),and resveratrol high-dose group(HR).The experimental results showed that after the resveratrol intervention,the learning and memory abilities of mice were improved,the latency of finding the platforms was shortened by 20.85%and 9.63%,respectively,for the low-dose group(LR)and the high-dose group(HR),the number of crossing the platforms increased by 53.63%and 47.57%,respectively,and the percentage of residence time in the target quadrant(where the platform was located)out of the total time increased by 103.69%and 53.68%,respectively.In addition,the body's antioxidant capacity increased,the level of total antioxidant capacity(T-AOC)and the activities of antioxidant enzymes(SOD and CAT)significantly increased(P<0.01),whilst the content of malondialdehyde(MDA)decreased(P<0.05).The morphology of hippocampus and amygdala nerve cells were regular,the inflammatory aggregation state was ameliorated,the expressions of inflammation-related genes(IL-1β,IL-6,and TNF-α)were significantly down-regulated(P<0.05),and the expressions of learning and memory related genes(BDNF,TrkB,and CREB)were significantly up-regulated(P<0.01).In conclusion,resveratrol could effectively ameliorate the oxidized casein-induced learning and memory impairments in mice,and the underlying mechanism may be related to the activation of the BDNF/TrkB/CREB signaling pathway to improve the antioxidant and anti-inflammatory capabilities of the body.