Comparative Analysis of the Key Genes of Triterpenoids in Rosa roxburghii Based on Metabolomics(LC-MS/MS)and Transcriptomics
HE Fengjin
YAN Yanfang
LIANG Qiancheng
TAO Xian
LI Haodong
LONG Tengxiang
LI Liangqun
DENG Tingfei
PAN Xiong
CHEN Faju
YANG Xiaosheng
Abstract：In this study,the biosynthesis pathway and regulatory mechanism of triterpenoids in Rosa roxburghii transcriptome analysis.The results showed that there were significant differences in triterpenoid content between cultivated and wild R.roxburghii varieties,with the cultivated variety exhibiting an approximately 30 wt.%higher triterpenoid content compared with the wild variety.Such a difference remained constant in the different growth stages of R roxburghii.Through high-throughput transcriptome sequencing technology,five key genes associated closely with the biosynthesis of triterpenoid were successfully identified in this research.These genes had high homology to genes such as CYP716A1,CPY734A1,CPY72A15,UGT74F2,and UGT85A2,suggesting their key roles in the synthesis of triterpenoid in R.roxburghii.In this study,the combined metabolomic and transcriptome analysis not only clarified the differences in the triterpenoid content of R.roxburghii and also successfully identified the key genes participating in the biosynthesis of triterpenoids,which offers fresh insights and potential molecular markers for the quality improvement and molecular breeding of R.roxburghii.
Keywords：Rosa roxburghiitriterpenoidsbiosynthesistranscriptome analysismolecular breeding
Publication Date：2025-06-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 129-136 )