Identification of Hypoglycemic Components in Cinnamon Using Cell-based Insulin Resistance and Enzyme Inhibition Models and Mass Spectrometry Analysis
DAI Yali
KAN Qixin
YANg Zhiying
LIANG Ziqi
LIN Qianru
ZHAO Lichao
CAO Yong
Abstract：Four different extracts of cinnamon powder were prepared using the solvents water,anhydrous ethanol,petroleum ether,and ethyl acetate.A model of insulin resistance was established using 3T3-L1 preadipocytes to evaluate the hypoglycemic effects of different solvent extracts.Next,α-amylase and α-glucosidase inhibition assays were performed to track and isolate the hypoglycemic components in the extracts.Thereafter,these components were identified.CCK-8 cell viability results showed that all four solvent extracts exhibited low cytotoxicity toward 3T3-L1 cells at concentrations below 100 μg/mL.In addition,the ethyl acetate extract demonstrated good hypoglycemic activity at concentrations of 10,20,and 50 μg/mL.In contrast,the ethyl acetate extract was less effective at α-glucosidase inhibition than the water extract,but displayed the highest α-amylase inhibitory activity.Based on these observations and our cell-based experiment results,the cinnamon extract in ethyl acetate was selected for further isolation.The F5 fraction obtained by silica gel column chromatography exhibited strong hypoglycemic effects,and the α-amylase and α-glucosidase inhibition rates were 66.08%and 98.21%,respectively.According to mass spectrometry analysis,the main constituents of the F5 fraction were trans-cinnamaldehyde and 4-methoxycinnamaldehyde,with relative molecular weights of 132.06 and 162.07,respectively.Trans-cinnamaldehyde and cinnamaldehyde exhibited specific hypoglycemic activities.These findings can serve as a valuable resource for further development and utilization of cinnamon as a hypoglycemic dietary supplement.
Keywords：cinnamon3T3-L1 preadipocyteshypoglycemicα-amylaseα-glucosidaseseparation and purification
Publication Date：2025-06-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 109-118 )