Antioxidant Capacity of Amomum tsao-ko Lipids and its Effects on Alloxan-induced Diabetes in Mice

HU Lianxia; XIE Libin; YU Longquan; CHEN Jia; ZHANG Wenna; XIAO Xiao; HAN Aiyun; LI Yanan; ZHANG Ning

doi:10.13982/j.mfst.1673-9078.2025.6.0405

Abstract：The antioxidant capacity of Amomum tsao-ko(A.tsao-ko)lipids and their effects on diabetes in mice induced by intraperitoneal injection of alloxan(ALX)(200 mg/kg)were investigated to provide support for both blood glucose reduction and organ damage prevention in diabetes.The antioxidant capacity of A.tsao-ko lipids was evaluated by measuring the scavenging activity against 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt(ABTS+)free radicals.Diabetic mice were fed a standard diet supplemented with(ALX+A.tsao-ko group)and without(ALX model group)A.tsao-ko lipids(500 mg/kg)for 28 days,and their body weight,blood glucose levels,and blood lipid parameters were measured.The results showed that A.tsao-ko lipids exhibit ABTS+and DPPH scavenging rates of 97.21%and 98.13%,respectively,at a concentration of 16 μg/mL.The weight loss in the ALX+A.tsao-ko group was not significant(P>0.05),but a decreasing trend in the blood glucose level was observed from 0 d to 7 d(P<0.05).The high-density lipoprotein(HDL)content in blood lipids in the ALX+A.tsao-ko group increased significantly(P<0.05),while the low-density lipoprotein(LDL)content decreased significantly(P<0.05)at the end of the feeding period(28 d).In conclusion,A.tsao-ko lipids have strong antioxidant activity and can effectively control weight loss in diabetic mice to reduce blood sugar,regulate blood lipids,alleviate organ damage,and support liver metabolism.Therefore,A.tsao-ko lipids can be developed and utilized as a hypoglycemic agent for diabetes and to control its deterioration.