Separation and Purification Processes of 2-O-α-D-Glucopyranosyl-L-Ascorbic Acid Prepared Enzymatically and Analysis of Its Antioxidant Activity
ZHANG Qiuying
SUN Jingjing
WANG Wei
JIANG Chengcheng
LIU Lili
HAO Jianhua
Abstract：In the study,2-O-α-D-glucosyL-L-ascorbic acid(AA-2G)was prepared using L-ascorbic acid andβ-cyclodextrin as substrates under the catalytic action of marine cyclodextrin glucosyltransferase MY20.The separation and purification process and antioxidant activity of AA-2G were examined.By using the cationic ion exchange resin SK1B and the anionic ion exchange resin WA30,the process parameters for separating AA-2G from anion exchange resin WA30 were optimized.The optimal process parameters were determined through response surface experiments:eluent concentration,0.15 mol/L;eluent flow rate,3.00 mL/min;eluent pH value 7.Under these conditions,the purity of AA-2G purified was 91.23 wt.％,with a recovery rate of 89.92 wt.％.On this basis,a multi-step purification process was established,in which increased the purity of AA-2G from the initial 50.31 wt.％to 95.62 wt.％,resulting in a 1.90-fold increase of purity and a recovery rate of 85.12 wt.％.In vitro antioxidant analysis of the prepared AA-2G revealed that the scavenging rates for superoxide anion,hydroxyl radical and DPPH radical were 91.63％,90.21％and 95.20％,respectively,indicating that the enzymatically prepared AA-2G possessed antioxidant activities.The study provides a theoretical basis for the development of high-purity AA-2G.
Keywords：2-O-α-D-glucopyranosyL-L-ascorbic acidL-ascorbic acidseparation and purificationanion-cation ion exchange resinmarine cyclodextrin glucosyltransferaseoxidation analysis
Publication Date：2025-05-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 68-78 )