Pomelo Peel Protein-tea Polyphenol Nanoparticle Applications and Elucidation of Their Transport Mechanism in a Caco-2 Cell Monolayer Model
KANG Jingling
LI Hanzong
LI Xinyi
LIANG Haobin
CHEN Zunxu
ZHONG Yuming
XIAO Gengsheng
WANG Qin
LIU Huifan
Abstract：To explore the absorption efficiency of pomelo peel protein-tea polyphenol nanoparticles(PP-TPNs)in vivo and elucidate potential food industry applications,the effects of PP-TPNs on cell viability,transepithelial electrical resistance,and alkaline phosphatase activity were evaluated using a Caco-2 cell monolayer model.PP-TPNs were also added to pomelo juice,carbonated beverages,and milk to determine their impacts on beverages by measuring color differences and polyphenol retention rates.At 400 μg/mL,unloaded nanoparticles and PP-TPNs both exhibit good biocompatibility,and respective cell viability rates are 88.83％and 84.27％.The respective unidirectional transport rates of PP-TPNs across a Caco-2 cell monolayer are 41.72％and 32.91％,and the bidirectional transport coefficient is 1.60.Application experiments indicate that after storage at 4℃ for 30 days,the respective polyphenol retention rates in pomelo juice and carbonated beverages with added PP-TPN are 82.42％and 79.62％.After heating at 95℃ for 1 h,the polyphenol retention rate in protein beverages with PP-TPNs remains at 91.89％.These studies indicate that PP-TPNs can easily be absorbed in the small intestine,primarily through active transport.PP-TPNs can also remain stable in beverages under adverse conditions such as high temperatures and light exposure.These findings provide a basis for further research on the absorption mechanism of PP-TPNs in the gut and offer insights to guide related application research.
Keywords：nanoparticlespomelo peel proteinstea polyphenolsCaco-2 cell monolayer modelbeverages
Publication Date：2025-04-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 14-24 )