Extraction Process Optimization and Antioxidant Activity Analysis of Green Radish Polysaccharide Based on Enzymatic Hydrolysis
TIAN Weina
ZHUANG Miaomiao
LI Jianrong
MA Wenting
LIN Ran
TAN Zhuohong
YU Zhangfu
SHEN Ronghu
GENG Xiong
Abstract：The extraction process for green radish polysaccharide(GRP)was optimized to improve the extraction yield of GRP by varying the enzyme activity,enzymatic hydrolysis time and temperature,and solid-solvent ratio in single-factor experiments using response surface methodology.The optimal GRP extraction process involved an enzyme activity of 285 U/g,an enzymatic hydrolysis temperature of 48 ℃,an enzymatic hydrolysis time of 59 min,and a solid-solvent ratio of 1∶21.The average yield of GRP extracted using this process was 5.34％.GRP was composed primarily of ten monosaccharides with a molar ratio of mannose∶ribose∶rhamnose∶glucuronic acid∶galacturonic acid∶glucose∶galactose∶xylose∶arabinose∶fuc ose=0.827∶0.365∶1.216∶1.519∶2.853∶66.215∶17.425∶0.295∶8.713∶0.574.Additionally,GRP presented excellent antioxidant capacity,and its scavenging rates for 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)radical,hydroxyl radical,and 1,1-diphenyl-2-trinitrophenylhydrazine radical were 91.14％,85.31％,and 62.73％,respectively,with half-maximal inhibitory concentrations of 10.78,8.42,and 17.40 mg/mL,respectively.The optimal GRP extraction process,monosaccharide composition and structure of GPR,and excellent antioxidant activity of GPR were determined,providing a reference for further research on the physiological activity of GRP and development of relevant health food.
Keywords：green radishpolysaccharideextraction processresponse surfaceantioxidant
Publication Date：2025-03-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 143-153 )