Characterization of Laccase from Neobacillus cucumis and Its Application in Industrial Dye Decolorization
XU Kaifeng
HUO Ying
ZHENG Suiping
Abstract：Laccase is widely used in the textile industry for dye decolorization,fabric surface modification,and textile bleaching.Screening for a laccase that is alkaline stable and suitable for textile dye decolorization is challenging.A novel alkaline laccase(LacNC)was obtained from Neobacillus cucumis and expressed in Escherichia coli.LacNC is a monomeric protein with a molecular weight of 58 ku.The optimum pH values for LacNC were 6.0,7.0,and 8.0 when 2,6-dimethoxyphenol,syringaldazine(SGZ),and guaiacol,respectively,were used as substrates.The optimal temperature for LacNC was 55 ℃ for the guaiacol substrate.The activity of LacNC remained more than 85％in the pH range of 5.0～11.0.After incubation at 40 ℃ for 1 h,the activity of LacNC was still higher than 80％.In addition,the decolorization rates of indigo carmine and malachite green by LacNC were more than 90％and 80％in 3 h,respectively,and the decolorization rate of reactive black B by LacNC reached 85％in 24 h.In this study,a new alkaline laccase demonstrating high decolorization efficiency for synthetic dyes under alkaline conditions was obtained.Hence,this laccase is an environmentally friendly candidate for treating wastewater in the textile industry.
Keywords：gene miningalkaline laccasealkali stabilitytextile industrydye decolorization
Publication Date：2025-03-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 107-114 )