Inhibition of UVB-induced Inflammatory Response and Regulation of Antimicrobial Peptide LL-37 by Polysaccharide Extracts from Saussurea laniceps
MA Wenrui
LIU Junchen
ZHANG Hongyan
LI Sunhua
LI Li
WANG Wei
Abstract：In order to study the effects of Saussurea laniceps polysaccharides(SLPs)on the expression of inflammatory factors and antimicrobial peptide LL-37 in UVB-induced keratinocytes,SLPs were extracted by the ethanol thermal reflux method,and SLPs at different concentrations were used to examine the inhibitory effect of COX-2(a key mediator of inflammatory pathway).A cell model of UVB irradiation-induced inflammation was established to determine the influence of SLPs on prostaglandin E2(PGE-2),TNF-α and IL-1β inflammatory factors,as well as the relationships of SLPs with LL-37 expression.An enzyme-linked immunosorbent assay(ELISA)and western blot analysis were used to detect the production of inflammatory factors and LL-37 antimicrobial peptide.The results showed that the inhibition rate of COX-2 was 82.41％at 1 000 μg/mL,and the expression of PGE-2,TNF-α and IL-1β inflammatory factors in HaCaT cells was significantly down-regulated at 100 μg/mL(P＜0.01).In addition,SLPs at 50 μg/mL and 100 μg/mL concentrations enhanced the expression of LL-37 antimicrobial peptide(P＜0.01),thereby down-regulating the expression of TNF-α and IL-1β inflammatory factors,then reducing skin inflammation.Conclusion:SLP can significantly inhibit the inflammatory response induced by UVB,and can further slow down the damage caused by inflammation to the skin by regulating LL-37 antimicrobial peptides,which has the potential to prevent skin inflammatory damage caused by UVB irradiation.
Keywords：Saussurea lanicepsultraviolet Binflammatory factorantibacterial peptide LL-37polysaccharide
Publication Date：2025-03-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 89-97 )