Anti-inflammatory Effect of Saikosaponin d on LPS-induced RAW264.7 Cell Inflammation
WANG Yanting
NIU Zhiqiang
LIU Ya'nan
LI Fu
HU Weicheng
ZHANG Ji
Abstract：A mouse model of lipopolysaccharide(LPS)-induced RAW264.7 cell inflammation was developed to investigate the in vitro anti-inflammatory mechanism of saikosaponin d(SSd).SSd demonstrated a significant inhibition of nitric oxide release from RAW264.7 cells at a concentration of 8 μmol/L(P＜0.01)and suppressed mRNA level expression of inflammation-related genes,including inducible nitric oxide synthase(iNOS),cyclooxygenase 2(COX-2),interleukin-6,and tumor necrosis factor-α,in a dose-dependent manner at concentrations of 4 and 8 μmol/L(P＜0.01).Western blot analysis revealed that SSd effectively reduced the protein level expression of iNOS and COX-2.SEM images confirmed that SSd alleviated LPS-induced morphological changes in RAW264.7 cells.Additionally,SSd inhibited the expression and phosphorylation levels of key proteins involved in inflammation-related signaling pathways,including toll-like receptor 4(TLR4),IκB kinase,nuclear factor KB(NF-κB),and glycogen synthase kinase-3β(GSK3β).These findings indicate that SSd strongly inhibits LPS-induced RAW264.7 cell inflammation by targeting the TLR4/NF-κB and GSK3β signaling pathways.These findings contribute to our enhanced understanding of the anti-inflammatory effects of SSd.
Keywords：saikosaponin dRAW264.7 cellsanti-inflammatory effecttoll-like receptor 4glycogen synthase kinase-3βnuclear factor κB
Publication Date：2025-03-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 80-88 )