Alleviating Effect of Fermented Barley Bran Extract on Lipid Deposition and Oxidative Stress in HepG2 cells

XIAO Mei; WU Xuemei; ZHU Ying; ZHANG Jiayan; XIAO Xiang; ZHAO Yansheng

doi:10.13982/j.mfst.1673-9078.2025.3.0196

Abstract：In this study,the effects of barley bran extract fermented by Lactiplantibacillus plantarum dy-1(Lp.plantarum dy-1)(FBBE)on lipid deposition and oxidative stress were investigated through a HepG2 high-lipid cell model.The results showed that,the protein content of barley bran did not change significantly after the fermentation with Lp.plantarum dy-1,but the protein with a relatively high Mw(around 52 ku)in barley bran was basically completely hydrolyzed,with the protein of 20～35 ku increasing significantly.The total sugar content was reduced by about 28％and the total phenolic content increased by about 42％.The FBBE treatment at 50 μg/mL decreased the TG content by 20.30％,whilst the polyphenols in the FBBE(FBBE-PP)at 5,10 or 15 μg/mL decreased the TG levels by 16.97％,27.41％and 34.92％,respectively,while reducing significantly the number of lipid droplets in the cells,indicating that polyphenol constituents were the effective components to alleviate lipid deposition in cells.After the FBBE-PP treatment at 10 μg/mL,the intracellular ROS content decreased by 23.45％and the SOD activity increased by 40.80％,compared with the model group.The FBBE-PP could up-regulate the expressions of ppar-α and cpt-1α,down-regulate the expressions of scd-1 and acc-1 to alleviate cell lipid deposition,while up-regulating the expressions of nrf-2,ho-1,sod1 and cat to enhance the antioxidative stress ability of cells.In conclusion,the fermentation with Lp.plantarum dy-1 could improve the lipid-lowering and antioxidant activities of barley bran,and its polyphenol components could significantly alleviate high-fat induced lipid deposition and oxidative stress in cells.