Mustard Leaf Extract Regulates Gut Microbiota and Wnt/JNK and NF-κB p65/COX-2 Signaling Pathways in A Mouse Colon Cancer Model
TIAN Yan
DU Haiping
CHEN Lanyan
ZHAO Lingyan
XU Yueju
ZHAO Yiying
DENG Fangming
Abstract：The therapeutic effects of mustard leaf extract,glucosinolate(GSL),on colon cancer were investigated.Total GSL content in mustard leaf was quantified using UV spectrophotometry.A colon cancer model associated with colitis was developed in Balb/c mice using azoxymethane and dextran sulfate sodium.The mice were divided into control,model,and low/high-dose mustard leaf GSL groups.After a 9-week trial,inflammatory cytokine levels and NF-κB p65/COX-2 and Wnt/JNK signaling pathway activities were analyzed.The gut microbiota was examined through high-throughput sequencing of 16S rRNA.Total GSL content in mustard leaf reached 127.11 μmol/g.Compared with the model group,the high-dose mustard leaf GSL group showed significant respective increases in weight and colon length(42.11％and 20.37％,respecitvely(P＜0.05)),a significant reduction in the number of colon tumors(P＜0.05),decreases in IL-6,TNF-α,and IL-17 expression in colon tissue by 46.88％,70.22％,and 20.43％respectively,a 98.55％increase in IFN-γ expression,and significant decreases in NF-κB p65,COX-2,Wnt,and JNK protein expression by 60.91％,15.57％,63.31％,and 96.58％,respectively(P＜0.05).The abundances of Bacteroides,Turicibacter,Ruminococcaceae UCG-014,Ruminiclostridium_5,Akkermansia,norank f Clostridiales vadinBB60 group,Prevotellaceae UCG-001,and Blautia were significantly altered by mustard leaf GSL,resulting in a restoration of a healthy microbial balance in the gut.In summary,leaf mustard GSL is a potential adjuvant therapy for colon cancer,as evidenced by its capacity to alleviate pathological conditions and modulate inflammatory cytokines,signaling pathways,and gut microbiota in mouse models of colon cancer.
Keywords：mustard leaf glucosinolate extractcolon cancergut microbiotaWnt/JNK signaling pathwayNF-κB p65/COX-2 signaling pathway
Publication Date：2025-03-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 1-11 )