Differential Analysis of Microbial Composition of Different Medium/high-temperature Daqu in Sichuan
WANG Yongjun
WEI Chunhui
LI Yanzhong
ZENG Bo
HUANG Zhiguo
HUANG Shuqin
REN Zhiqiang
Abstract：In order to study the composition of caproic acid-producing bacterial community in Medium/high-temperature Daqu,four different medium/high-temperature Daqu in Sichuan were used as the research objects.After the enrichment with the enrichment medium,the metagenomic DNA of Daqu and Daqu enrichment liquid samples were subjected to high-throughput sequencing with 16S rRNA gene amplicons,and the composition of caproic acid-producing bacterial community in Daqu was analyzed.The results showed that the diversity and abundance of the potential caproic acid-producing bacterial community in Daqu increased after the enrichment.The potential dominant caproic acid-producing bacteria(relative abundance>1%)in Daqu included Clostridium_sensu_stricto_18(2.19%～69.00%),Clostridium_sensu_stricto_15(1.76%～7.65%)and a small amount of Clostridium_sensu_stricto_12,Clostridium_sensu_stricto_1,Clostridium_sensu_stricto_10,Clostridium_sensu_stricto_7 and Caproiciproducens.Among them,in terms of the main potential caproic acid-producing bacterial community,Q1 Daqu was dominant with Clostridium_sensu_stricto_18,and Clostridium_sensu_stricto_15;Q2 Daqu was dominant with Clostridium_sensu_stricto_1,Caproiciproducens,and Clostridium_sensu_stricto_18;Q3 Daqu was dominant with Clostridium_sensu_stricto_18,and Caproiciproducens;Q4 Daqu was dominant with Clostridium_sensu_stricto_18,and Clostridium_sensu_stricto_1.The results showed that there were differences in the composition of caproic acid-producing bacterial communities in different medium/high-temperature Daqu in Sichuan.The preliminary analysis was carried out to provide a theoretical basis for the directional study of caproic acid-producing bacteria in Daqu,and also provide a direction for the preparation of high-quality Daqu to promote the quality improvement of Nongxiangxing liquor.
Keywords：caproic acid-producing bacteriamedium-high-temperature daquanaerobic enrichmenthigh-throughput sequencing
Publication Date：2025-01-14
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 89-96 )