Growth-promoting Effect of Cyclic Dipeptide Cyclo(Leu-Pro)on Lactobacillus plantarum
TAN Xu
WU Zhongkun
XIA Di
GAO Ning
WANG Li
GAO Yao
GUO Xinmei
LIU Xuecong
WANG Yuanliang
Abstract：In this study,it's the effect of Cycle(Leu Pro)on the growth of Lactobacillus plantarum was investigated.Lactobacillus Plantarum T1 was used as the research object,and high-performance liquid chromatography(HPLC)was used to preliminarily characterize and quantify the Cyclo(Leu-Pro)in the fermentation broth of Lactobacillus Plantarum T1.Lactobacillus Plantarum T1 was respectively inoculated in the MRS broth medium without or with different mass concentrations(0.5,1,5,10 μg/mL)of Cyclo(Leu-Pro)for 18 h.The growth curves and viable bacterial counts of Lactobacillus plantarum T1 under different culture conditions were determined by the 96-well method and plate counting method.The results showed that Cyclo(Leu-Pro)at different mass concentrations had significant growth-promoting effects on the growth of Lactobacillus plantarum T1,among which,1 μg/mL Cyclo(Leu-Pro)had the greatest growth-promoting effect and could increase the viable counts to 8.73×1010 CFU/mL,which was an order of magnitude higher than the control.Under different stress conditions,1 μg/mL Cyclo(Leu-Pro)could shorten the lag period and increase the growth rate of Lactobacillus plantarum T1,and its viable count and biomass increased significantly(P<0.05).In summary,Cyclo(Leu-Pro)promoted the growth of Lactobacillus plantarum T1 and enhanced its stress resistance.This will favor the application of Lactobacillus plantarum T1 as a starter in the field of food fermentation.
Keywords：cyclo(Leu-Pro)Lactobacillus plantarumgrowth promotionproliferation
Publication Date：2025-01-14
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 81-88 )