Ginsenoside Rg2 Alleviates Lipopolysaccharide-induced Inflammation of RAW264.7 Cells by Regulating the PI3K/AKT/NF-κB Signaling Pathway
XIE Jing
YUAN Qiaoyun
HE Ziliang
YANG Guigui
GAO Xiang
ZHANG Wenxia
ZHANG Ji
HU Weicheng
Abstract：The anti-inflammatory effects of ginsenoside Rg2 were investigated based on a bacterial lipopolysaccharide(LPS)-induced inflammation model of RAW264.7 cells.The expression levels of inflammation-related genes and proteins in the model were analyzed using PCR,RT-qPCR,and western blotting.The results demonstrated that ginsenoside Rg2 dose-dependently inhibited the release of nitric oxide from inflammatory cells(P＜0.05)and transcription levels of inflammation-related genes,including inducible nitric oxide synthase,cyclooxygenase-2,tumor necrosis factor-α,and interleukin-1β(P＜0.05).Scanning electron microscopy analysis showed that ginsenoside Rg2 improved the morphology of inflammatory RAW264.7 cells after LPS induction.Western blotting results revealed that ginsenoside Rg2 inhibited the phosphorylation of PI3K,PDK1,AKT,and GSK-3β and prevented NF-κB from entering the nucleus.These results suggest that ginsenoside Rg2 has an anti-inflammatory effect and can reduce LPS-induced inflammation of RAW264.7 cells via the PI3K/AKT/NF-κB signaling pathway.The findings of this study improve the understanding of the anti-inflammatory activity of ginsenoside Rg2 and associated mechanism and provide a foundation for the development of related functional foods.
Keywords：ginsenoside Rg2anti-inflammationRAW264.7 cellsPI3K/AKT/NF-κB signaling pathway
Publication Date：2024-12-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 1-9 )