Process Optimization of the High-density Fermentation with Lactiplantibacillus plantarum J26 and Its Application to the Fermentation of Soy Milk
ZHENG Yaping
WANG Junhao
WANG Chenchen
ZHANG Yu
JIANG Yujun
MAN Chaoxin
Abstract：Lactiplantibacillus plantarum J26 was used as the research object.The carbon and nitrogen sources were screened using the Biolog GEN 111 Microstation automated microbial identification system.After optimization,a mixture of 25 g/L maltose and glucose(1∶1)was used to replace the carbon source in the MRS medium,and a mixture of 10 g/L beef paste and yeast powder(1∶1)was used to replace the nitrogen source in the MRS medium.The optimal fermentation conditions for the strains determined by single factor experiment were as follows:initial pH of the medium,5.6;fermentation temperature,33 ℃;inoculation amount,4％.Under these conditions,a 5-liter fermenter was used for high-density fermentation.Lactiplantibacillus plantarum J26 reached the stable stage after 14 h of fermentation,and the viable cell count could reach 4.35× 1010 CFU/mL.Because Lactiplantibacillus plantarum J26 has a high viable cell count and high fermentation activity,thus can be used as a starter for the fermentation of soy milk.Fermented soy milk with a good viable bacterial count(3.56x108 CFU/ml),water-holding capacity(48.12％),and textural characteristics was obtained.By optimizing the high-density fermentation process,the number of viable bacteria in the fermentation broth can be effectively increased.This provides a theoretical basis for the application of high-density fermentation with Lactiplantibacillus plantarum J26 and the industrialization of fermented soy milk.
Keywords：Lactiplantibacillus plantarum J26high density fermentationprocess optimizationsoy milk
Publication Date：2024-10-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 154-164 )