Structure and Bioactivities of a Carboxymethyl Polysacchairde from Pachyman
LIU Xiao-fei
HU Shuang-fei
ZHANG Xue-wu
Abstract：A carboxymethyl polysaccharide from Pachyman was isolated and purified using DEAE-52 cellulose column, Sephadex-G200 column and Sephadex-G150 column in the work. The structure of this polysacchairde was identified and its anti-proliferation activity and anti-inflammation activity investigated. This polysaccharide is a (1→3)-β-D-glucan with an average molar mass of 20.96×104 u, which also has a little (1→2) glucosidic bonds. Its monosaccharide composition only contained D-glucose and the triple helical structure did not exist. This polysacchairde exhibited a strong anti-proliferation effect on HT-29, Hep G-2, SGC-7901, MCF-7 and A549 cells, with IC50 values of 140.5 μg/m L, 264.3 μg/m L, 102.5 μg/m L, 256.4 μg/m L and 313.2 μg/m L, respectively. In addition, this polysaccharide exhibited an inhibitory effect on LPS-induced RAW264.7 cells, but could activate RAW264.7 cells in the absence of LPS. The findings would be of great importance for the development of Pachyman polysaccharide-based health foods and drugs.
Keywords：Carboxymethyl polysacchairdestructureanti-proliferation activityanti-inflammation activity
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 42-49,7 )