B Cell Epitopes Analysis and Prokaryotic Expression of PGIP in Citrus
CHEN Bo
LUO Qing-hua
TAN Ya-qin
YAN Hui-qing
Abstract：Citrus is an important horticulture. However consumption of citrus fruits could cause allergen for some people. With the method of Jameson-Wolf, Kyte-Doolittle, Emini and Karplus-Schulz, the antigenicity, hydrophilicity, protein surface accessibility and flexibility of citrus allergen protein PGIP (polygalacturonase inhibitor protein) were predicted. Meanwhile PET-28 a-PGIP vector was constructed, and then transfer to Escherichia coli. The target gene was induced and expressed. The results showed: When the secondary structure of amino acid was β-angle and irregular curl, the antigen index＞0, the flexibility＞0, the hydrophilicity＞0, the protein surface accessibility＞1 amino acid region, the sequences of the B cell epitope binding amino acid were 1922, 3741, 4952, 6869, 117120, 163164, 173177, 221226, 236240. By estimating the amino acid region, it provides support for finding the best possible region of the B cell epitope of citrus allergen protein PGIP, and also provides a theoretical basis for the further study of citrus allergen protein PGIP.
Keywords：citruspolygalacturonase inhibitory proteincell epitope
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 18-22,29 )