Structural Identification of Phenolic Constituents from Phyllanthus emblica L.and Their Inhibitory Activity on a-glucosidase
LIU Wei
LI Ming-xi
WANG Jun-long
WAN Chun-peng
Abstract：The phenolic constituents in the hypoglycemic active fraction of the methanol extract of Phyllanthus emblica L and their inhibitory activity on α-glucosidase were studied.Using methanol soaking extract Phyllanthus emblica L,the methanol extract (PE) was chromatographed on a D101 macroporous resin column to obtain three fractions (PEA-PEC).Hypoglycemic activity test showed that 30％ ethanol elution part (PEA) was the active fraction.PEA was further isolated and purified by column chromatography of Sephadex LH-20,ODS and semi-preparative HPLC columns.The structures of the isolated compounds were identified according to the physicochemical properties,1H-NMR,13C-NMR and high resolution mass spectral analyses as well as relevant references.The compounds were tested for their in vitro inhibitory activity on α-glucosidase.Seven phenolic compounds were isolated from PEA fraction and identified as gallic acid (1),methyl gallate (2),1-O-galloyl-β-D-glucose (3),1,6-di-O-galloyl-β-D-glucose (4),corilagin (5),mucic acid-1,4-Lactone-2-O-gallate (6),mucic acid-2-O-gallate (7).All compounds except compound 2 showed good inhibitory activity on α-glucosidase.Gallic acid derivatives were the active constituents that P.emblica L.inhibited the activity of a-glucosidase.
Keywords：Phyllanthus emblica L.chemical constituentsphenolic constituentsinhibitory activity of α-glucosidase
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 50-55 )