Antibacterial Activity of Volatile Oil and Residue Extract from Lonicera Japonica

RUAN Zhi-yang; WANG Zhao-mei; LI Jia-jia; WEI Xi-qiong

doi:10.13982/j.mfst.1673-9078.2017.10.012

Abstract：Lonicerajaponica,obtained from Shandong and Guangdong,was used as the raw material in this study.The volatile oil was separated by CO2 supercritical fluid extraction,and afterwards the water-soluble and the water-insoluble subfraction were prepared from the residue after volatile oil isolation.The chemical composition of volatile oil was analyzed by GC-MS and high performance liquid chromatography was used to determine the content of chlorogenic acid and galuteolin in residue extract of Lonicerajaponica.The anti-bacterial activities of volatile oil and extract were investigated by disk diffusion assay and minimum inhibitory concentration (MIC).The main components of Lonicerajaponica volatile oil were fumaronitrile (61.5％) and amyl acetate (37.7％),and the content of lorogenic acid and galuteolin in water-soluble residue extract from Shandong province was the highest among the 4 residue extracts.The Lonicerajaponica volatile oil and subfractions of ethanol extract exhibited different inhibitory effects on the six pathogens.The antimicrobial effect of the two extracts on gram-positive bacteria was better than that of gram-negative bacteria.The antibacterial effect of the volatile oil in Lonicera japonica from Shandong was the strongest with the MIC of 64 μL/mL and the diameter of inhibition zone of 9.3 mm.Meanwhile,the water-soluble subfraction of Lonicerajaponica from Guangdong showed a significantly high antibacterial effect on bacillus subtilis with the diameter of inhibition zones above 15mm and MIC value of 16 pL/mL.There was no significant difference in the antibacterial activity of the extract in Lonicerajaponica from Guangdong and Shandong.