Hypoglycemic Activity of Phyllanthus emblica L extracts and Analysis of its Main Components
LI Ming-xi
HUANG Wei-feng
YAO Liang-liang
WAN Chun-peng
Abstract：The effects of Phyllanthus emblica L extract on mRNA expressions of GLUT-2 (glucose transporter type 2) and PPARγ (peroxisome prolifemtor-activated receptor-γ) and the activities of PPRE (peroxisome prolifemtor response elemem) and NF-κB in LO2 cells were studied to elucidate the hypoglycemic mechanism and the main active components of this extract.The methanol extracts of P.emblica L were first isolated using a D101 macroporous resin column to yield three fractions.Real-time polymerase chain reaction (PCR) and luciferase reporter gene methods were applied to study the effects of the extracts ofP emblica L and theit active components on the mRNA expressions of GLUT-2 and PPARγ and the activities of PPRE and NF-κB.The extracts of P emblica L significantly enhanced the mRNA expression of GLUT-2 and PPARγ and activity of the PPRE reporter gene (p＜0.05),inhibited the lipopolysaccharide (LPS)-induced inflammation (p＜0.05),and reduced the activity of the NF-κB reporter gene;the inhibitory effect was dose-dependem (p＜0.05).The 30％ ethanol-eluted fraction from the macroporous resin column was the active fraction.Sephadex LH-20,C 18 (ODS),semi-preparative high performance liquid chromatography (HPLC) columns were used,and one main componem was isolated and identified as gallic acid.P.emblica L might exert its hypoglycemic effect by increasing the expressions of GLUT-2 and PPARγ and inhibiting the inflammation-related pathway.Gallic acid may be the major active component.
Keywords：Phyllanthus emblica Lglucose transporter 2 (GLUT-2)peroxisome proliferator-activated receptor γ (PPARγ)peroxisome proliferator response element (PPRE)nuclear factor-κB (NF-κB)
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 96-101 )