Synergistic Protective Effects of Casein Hydrolysate and Vitamins A, C, or E against Ethanol-induced Damage in Hepatocyte HHL-5 Cells
PANG Jia-nan
CHEN Fang-fang
MA Chun-min
QI Li-jia
LI Tie-jing
Abstract：Casein hydrolysate (CH) can be used to treat and repair hepatocytes damaged by ethanol-induced oxidative stress.The aim of this study was to evaluate the synergistic effects of vitamin A,vitamin C,or vitamin E with CH to protect the liver cells from oxidative damage in vitro.Previous studies have shown that CH has no obvious toxicity to liver cells (HHL-5),and can even promote proliferation.The results showed that 300 mmol/L ethanol induced obviously oxidative damage to HHL-5 cells,and CH significantly enhanced the viability of these cells.Combining vitamin A (0.0344 mg/mL),vitamin C (0.0881 mg/mL),or vitamin E (0.1077 mg/mL) with CH significantly enhanced the viability of liver cells damaged by ethanol-induced oxidation.The concentration of CH for the optimum synergistic effect was selected using a cell counting kit-8 (CCK-8) assay and determination of lactate dehydrogenase (LDH) content in the culture medium.This concentration was verified through the determination of intracellular content of malondialdehyde (MDA) and reactive oxygen species (ROS).CH at 2 mg/mL alone exhibited a certain restorative effect on cells damaged by ethanol-induced oxidation,and the synergistic protective effects of combining CH at this concentration with vitamin A,vitamin C,or vitamin E were significantly higher (p＜0.05).
Keywords：casein hydrolysateethanoloxidative stresssynergistic protection
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 32-37 )