Separation and Purification of Straight-chain Peptide Metabolites Produced by Bacillus subtilis SC-2
ZOU Yuan-jun
SONG Xin-pei
ZHENG Yi-min
LUO Jie
ZHONG Meng
SONG Ting
XU Xiu-ying
Abstract：In order to study the main antimicrobial substances in the fermentation liquid of Bacillus subtilis SC-2,clinical strains of pathogenic methicillin-resistant Staphylococcus aureus (MRSA),Propionibacterium acnes,and Candida albieans were used as indicator strains,antimicrobial activity was measured using the agar diffusion method,and the crude extracts of SC-2 fermentation liquid were separated and purified.The crude extract containing antimicrobial substances was separated by silica gel column chromatography,Sephadex LH-20 gel column chromatography,and high performance liquid chromatography (HPLC) to yield a monomeric compound,GNH-2,with antibacterial activity.This substance showed broad-spectrum antimicrobial activity.At a concentration of 1.0 mg/mL,the GNH-2 inhibition zone diameters against Candida albicans,Escherichia coli,and E.coli 251 were 34±0.30 mm,18±0.15 ram,and 19±0.10 mm,respectively.The GNH-2 inhibition zone diameters against S.aureus,MRSA,and Propionibacterium acnes were 28±0.10 mm,30±0.10 mm,and 56±0.35 rmm,respectively,which were better than those of vancomycin at the same concentration.The results of ninhydrin coloration preliminarily suggested that GNH-2 was a straight-chain peptide.This work provides the basis for subsequent structure confirmation and new drug development.
Keywords：Bacillus subtilisseparation and purificationantimicrobial activitystraight chain peptides
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 85-90 )