Preliminary Study of Seleno-chitosan-induced Apoptosis in A549 Cells through a Mitochondrial Signaling Pathway
ZHANG Shao-jing
LIU An-jun
WANG Peng-fei
ZHENG Guo-qiang
Abstract：The effects of seleno-chitosan (SC) on cell proliferation inhibition and apoptosis in human non-small-cell lung cancer A549 cells were investigated,and the differential expression of proteins in SC-treated and untreated cells were determined.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to determine the inhibitory effect of SC on the proliferation of A549 cells.Morphological changes in the A549 cells after Hoechst 33342/PI staining and the opening of the mitochondrial permeability transition pore (MPTP) after calcein staining were observed by laser scanning confocal fluorescence microscopy.The effect of SC on mitochondrial membrane potential (△ψm) was analyzed by flow cytometry (FCM).Furthermore,two-dimensional gel electrophoresis (2-DE)combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) was used to identify the differential expression of apoptosis-related proteins.The results showed that SC could significantly suppress the proliferation of A549 cells in a time-and concentration-dependent manner.Morphological changes of A549 cells could be induced by SC;the cell shape became irregular and chromatin condensation and margination occurred.After SC treatment,the MPTP of A549 cells was gradually opened.Additionally,two differentially expressed proteins,prohibitin isoform 1 and heat shock protein,were identified by MALDI-TOF.
Keywords：seleno-chitosanA549 cellsapoptosis
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 46-51 )