Analysis of the Volatile Components of Lithocarpus polystachyus Rehd.and a Preliminary Study of Their Anticancer Activity
YAN Ni
WANG Zuo
GAO Xiong
ZHANG Yuan-yuan
LIN Xiao-rong
LI Bin
CHEN Zhong-zheng
Abstract：Lithocarpus polystachyus Rehd.was selected as a starting material to extract volatile components using simultaneous distillation extraction (SDE) method.The composition of volatile components was analyzed by gas chromatography-mass spectrometry (GC-MS).Subsequently,the anticancer activities of the volatile components and major monomeric substances of Lithocarpus polystachyus Rehd.were studied using mouse hepatoma cells (Hepa1c1c7).A cell viability of greater than 50％ and quinone reductase (QR) induction activity greater than or equal to 2 were set as the activity indicators.The results showed that a total of over 80 types of major volatile compounds were identified by the GC-MS analysis,accounting for 92.56％ of the total volatiles.Ketones,alcohols,and aldehydes were the major chemical types.Among them,geranylacetone,β-ionone,nonanal,and spathulenol were the most abundant volatile components.The measurement of anti-cancer activity indicated that the induced QR activity was amplified when the concentration of volatile components ofLithocarpus polystachyus Rehd.was (27.50±0.10) μg/mL.And the concentrations of linalool,geranylacetone,and nonanal,the three main volatile components that enhanced the induced QR activity,were (54.53±0.10) μg/mL,(283.10±0.10) pg/mL,and (297.77±0.12) μg/mL,respectively.
Keywords：Lithocarpus polystachyus Rehdvolatile componentscomposition analysisanti-cancer activity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 35-39,73 )