Effect of Lactobacillus plantarum 35 on Escherichia coli-induced IL-8 Production and E.coli Adhesion on HT-29 Cells
GUO Yuan-ji
TUO Yan-feng
MOU Guang-qing
JIANG Shu-juan
QIAN Fang
LIU Peng
SHEGN Xiao-fang
Abstract：The adhesive ability of eight strains of Lactobacillus plantarum on the HT-29 intestinal epithelial cell line,and the inhibitory effect of the strain with the highest adhesive ability and its exopolysaccharides (EPS) on Escherichia coli-induced IL-8 production and E.coli adhesion on HT-29 cells were investigated.The results indicated that the eight Lactobacillus strains adhered to HT-29 cells to different degrees,and L.plantarum 35 had the highest adhesive ability.L.plantarum 35 could protect HT-29 cells from E.coli adhesion by displacement,competition,and exclusion,with inhibition rates of 30％,33％,and 59％,respectively.At an active concentration of 500 μg/mL,EPS could inhibit E.coli adhesion by 32％.L.plantarum 35 could inhibit E.coli-induced IL-8 production by HT-29 cells by displacement,competition,and exclusion with inhibition rates of 3％,28％,and 40％,respectively.EPS could inhibit E.coli-induced IL-8 production by HT-29 cells in a dose dependent manner,with the highest inhibitory rate of 50％ at 500 μg/mL concentration.L.plantarum 35 had no effect on IL-10 production by HT-29 cells.The results showed that L.plantarum 35 has a potential probiotic function and may inhibit inflammatory bowel disease induced by E.coli.
Keywords：LactobacillusHT-29 cellsE.coli ATCC25922adhesion
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 14-21 )