ABTS Radical Scavenging Activity of Theaflavins Combined with Fungal Polysaccharides
ZHANG Nai-xun
YIN Hong-li
LIU Ran
YU Mei-hui
LI Ting-ting
WANG Zhen-yu
Abstract：The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities of theaflavins (TFs) alone and combined with Auricularia auricular polysaccharides (AAP) and Ganoderma lucidum spore polysaccharides (GLP) were compared in this paper.The results of ABTS radical scavenging experiments were analyzed using the Chou-Talalay combination index (CI),and the scavenging effects,combination index (CI),and dose-reduction index (DRI) of TFs,AAP,and GLP,alone and in combination,were evaluated.The half maximal inhibitory concentration (IC50) values ofTFs,AAP,and GLP were 20.60 μg/mL,2.49 mg/mL,and 0.93 mg/mL,respectively,while the IC50 values of a combination of TFs and AAP (mass ratio 1:3) and a combination of TFs and GLP (mass ratio 1∶3) were 74.04 μg/mL and 58.92 μg/mL,respectively.CI analysis indicated that with the clearance rate in the range of 5～97％,the C1 values of TFs with GLP were ＜ 1 and exhibited a downward trend,suggesting a synergistic effect between TFs and GLP.DRI analysis demonstrated that with the clearance rate in the range of 5～97％,the DRI values of TFs with GLP were greater than 1.Inconsistent results were obtained in the above analyses for a combination of TFs and APP.Therefore,there was a synergistic effect between TFs and GLP for scavenging ABTS free radicals,but no similar effect was found between TFs and APP.
Keywords：fungal polysaccharidestheaflavincombinationantioxidant activity
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 21-28,34 )