Optimization of Enzymolysis of Chitosan-Fe2+ by Response Surface Methodology
Abstract：A new method for controllable preparation of chitosan oligosaccharides was developed by enzymolyzing the coordination complex of chitosan and Fe2+ (Chitosan-Fe2+,CTS-Fe2+) with papain in this study. The effects of four process parameters including pH, temperature, substrate concentrations and enzyme-substrate ratio on the enzymolysis of CTS-Fe2+ with papain were investigated by using the response surface methodology (RSM). To reveal the viscosity decrease percentage (VDP) of the enzymolyzed CTS-Fe2+, a second order polynomial mathematic model of the reaction was built according to the analysis results. The optimum pH value, temperature, substrate concentration and enzyme-substrate ratio were 4.46, 44.95 ℃, 1.97％ and 1.75％, respectively.
Keywords：papainChitosan-Fe2+response surface methodologyoptimum process parameters
Publication Date：2008-01-01
Pages：4( 787-790 )