Simultaneous determination of aconitine-type alkaloids and rhodojaponin Ⅱ in Weikang Gel by HPLC-MS/MS
Chen Weiwei
Li Xianliu
Liu Yanxia
Lin Shuilan
Huang Haifu
Zhang Jun
Abstract：Objective To establish an efficient high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the simultaneous determination of six aconitine-type alkaloids(benzoylhypacoitine,benzoylmesaco-nine,benzoylaconine,hypaconitine,mesaconitine,aconitine)and rhodojaponin Ⅱ in Weikang Gel.Methods Chromato-graphic separation was performed on an Agilent InfinityLab Poroshell 120 EC-C18 column(4.6 mm × 150 mm,4 μm)using acetonitrile-0.1％formic acid in water as the mobile phase with gradient elution;column temperature was 30 ℃ and flow rate 0.45 mL/min.Detection was carried out by electrospray ionization in positive ion mode using multiple reaction monito-ring.Results The reference solution was prepared with a blank matrix,and the linear relationship between the six aconitine alkaloids and rhodojaponin Ⅱ was good in their respective ranges(r≥ 0.999 0).Precision,stability,and repeatability met method validation requirements.Mean spiked recoveries ranged from 81.56％to 107.08％,with relative standard deviations(RSDs)of 1.75％-4.78％.Conclusion The developed HPLC-MS/MS method overcomes matrix effects,offers high sen-sitivity and specificity,and can provide an experimental basis for quality control strategies aimed at balancing efficacy and toxicity of Weikang Gel.
Keywords：Weikang GelHPLC-MS/MSAconitine-type alkaloidsRhodojaponin ⅡContent determination
Publication Date：2025-09-20
Online Publishing Date：2026-01-06(First online date of this platform, not the publication date of the document)
Pages：9( 25-33 )