Relationship between serum IL-8 and IL-10 levels and clinicopathological features and sentinel lymph node metastasis in breast cancer patients
Zhang Yan
Sun Xin
Liu Songling
Yu Xi
Wang Zhiqun
Wang Xia
Chen Anqing
Abstract：Objective To explore the relationship between serum interleukin-8(IL-8)and interleukin-10(IL-10)expression levels and clinicopathological features and sentinel lymph node(SLN)metastasis in breast cancer patients,providing a refer-ence for clinical decision making.Methods From November 2022 to November 2024,152 patients with breast cancer trea-ted at a hospital in Shandong were enrolled.Clinical data were collected and serum IL-8 and IL-10 levels were measured.Se-rum IL-8 and IL-10 levels were compared across patients with different clinicopathological features.SLN biopsy was per-formed and patients were assigned to metastasis and non-metastasis groups.Receiver operating characteristic(ROC)curves were used to evaluate the predictive value of serum IL-8 and IL-10 for SLN metastasis.Univariate and multivariate logistic stepwise regression analyses were used to identify risk factors for SLN metastasis.Results Patients with tumor diameter ≥2 cm had higher serum IL-8 and IL-10 levels([72.18±19.91]ng/L and[66.65±16.53]ng/L)than those with tumor di-ameter＜2 cm([65.27±16.82]ng/L and[58.91±15.07]ng/L;t=2.192,2.887,P=0.030,0.004).Poorly differen-tiated tumors were associated with higher IL-8 and IL-10 levels([76.51±21.73]ng/L and[70.60±17.04]ng/L)ver-sus moderate-well differentiated tumors([64.95±16.74]ng/L and[59.16±15.81]ng/L;t=3.697,4.238,P＜0.001).Patients with vascular invasion had higher IL-8 and IL-10([73.26±19.43]ng/L and[67.82±14.59]ng/L)than those without vascular invasion([66.62±17.21]ng/L and[60.46±17.01]ng/L;t=2.232,2.824,P=0.027,0.005).Serum IL-8 and IL-10 levels in the metastasis group were significantly higher than in the non-metastasis group(P＜0.05).ROC a-nalysis showed that combined detection of serum IL-8 and IL-10 for predicting SLN metastasis yielded an area under the curve(AUC)of 0.904(0.859-0.954),which was significantly higher than IL-8 alone(AUC=0.769[0.724-0.819])and IL-10 alone(AUC=0.817[0.772-0.867];Z=10.247,9.612,P＜0.05).Univariate analysis indicated that tumor diameter,histological differentiation,vascular invasion,IL-8,and IL-10 were associated with SLN metastasis(P＜0.05).Multivariate analysis identified low differentiation(OR=2.497,95％CI:1.292-4.824),vascular invasion(OR=2.784,95％CI:1.599-4.849),IL-8 ≥71.50 ng/L(OR=3.165,95％CI:1.857-5.393)and IL-10 ≥66.40 ng/L(OR=3.367,95％CI:2.007-5.649)as independent risk factors for SLN metastasis in breast cancer patients(P＜0.05).Con-clusion Serum IL-8 and IL-10 are highly expressed in breast cancer patients,and their levels are closely associated with tumor di-ameter,histological differentiation,vascular invasion,and SLN metastasis.Serum IL-8 and IL-10 can serve as clinical indicators for predicting SLN metastasis in breast cancer,and combined detection shows superior predictive performance.
Keywords：Breast cancerInterleukin-8Interleukin-10Sentinel lymph node metastasis
Publication Date：2025-08-20
Online Publishing Date：2025-12-09(First online date of this platform, not the publication date of the document)
Pages：7( 51-57 )