Analysis of molecular transmission networks and pretreatment drug resistance in newly reported HIV/AIDS cases in Jiulongpo district,Chongqing
He Wei
He Jingchun
Chen Hao
Pan Haiying
Wu Lingli
Liu Yangji
Tao Ran
Abstract：Objective To understand the molecular transmission network characteristics of newly reported HIV/AIDS cases in Jiulongpo District,Chongqing,and provide references for targeted AIDS interventions.Methods Newly reported HIV/AIDS cases in Jiulongpo District(2022-2024)were enrolled.Blood samples and demographic data prior to antiviral therapy were collected.After extraction,pol region amplification,sequencing,and sequence alignment,Gene sequences were ana-lyzed for viral subtypes and resistance.The HIV-TRACE tool was used to construct and analyze molecular transmission net-works.Results A total of 222 valid gene sequences were obtained:CRF07_BC subtype(53.60％,119/222),CRF08_BC subtype(24.77％,55/222),CRF01_AE subtype(13.96％,31/222),and other subtypes(7.66％,17/222).Using a 1.5％genetic distance threshold to establish the molecular transmission network,the network entry rate was 37.84％(84/222),forming 31 transmission clusters.The largest cluster comprised 14 males aged ≥50 years.Significant differences in network entry rates were observed across subtypes,age,education,occupation,marital status,and transmission routes(P＜0.05).Multivariate analysis showed age ≥50 years(OR=3.303,95％CI:1.363-8.006)and heterosexual trans-mission(OR=4.656,95％CI:1.249-17.347)were associated with higher clustering risk as compared with age＜50 years and homosexual transmission.Pretreatment drug resistance rate among these participants was 5.86％(13/222).Con-clusion CRF07_BC,CRF08_BC,and CRF01_AE subtypes are the dominant HIV subtypes in Jiulongpo District.Integra-ting molecular transmission networks with epidemiological field investigation is conducive to identifying outbreak linkages and population/regional clusters.Sustained molecular transmission network surveillance and targeted interventions are es-sential for curbing HIV transmission.
Keywords：AIDSMolecular transmission networkGenetic subtypeDrug resistanceTargeted intervention
Publication Date：2025-08-20
Online Publishing Date：2025-12-09(First online date of this platform, not the publication date of the document)
Pages：7( 14-20 )