Ex vivo H1-MRS for evaluation of human gastrointestinal carcinoma with 7.0 T NMR spectrometer
OU Yangqing
HUANG Ranran
XIE Lei
MA Shuhua
ZHANG Jiong
XIAO Yifeng
Abstract：Objective The aim of the present study was to examine the metabolic profile of normal and tumoral gastric cell with ex vivo high resolution magnetic resonance spectrometer (7.0 T NMR) to get information on the molecular steps involved in gastric carcinogenesis and the identification of biochemical markers useful for the development of in vivo MRS methodologies to diagnose gastric carcinoma in clinical situation. Methods The Normal gastric mucosa epithelial cell (GES-1) and gastric cancer cell (BGC-823) line were collected and analyzed with NMR, then the concentrations of fatty acids were calculated. Results The spectrums of the GES-1 and BGC-823 showed that presences of differently distributed fatty acids were the markers of a physiological condition. The GES-1 cells had a higher concentration lipid contents than GES-1 did in two independent-samples t tests (Lipid methyl:2.796 ±0.975 vs. 0.943 ±0.629, P<0.001;Lipid methylene 14.660 ± 5.106 vs. 4.473 ±2.296, P<0.001;N-acetylneuraminic acid 2.638 ±1.080 vs. 0.528 ±0.734, P<0.001). Conclusion Ex vivo 1H-MRS is a viable and powerful probing means for molecular information in human normal and tumoral cell lines. This research may constitute the basis for a biochemical classification of cancer cell using in vivo MRS for clinical purposes.
Keywords：Gastric carcinomaCell lineMagnetic resonance spectroscopyBiomarker
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 409-412 )