Evaluation of renal perfusion in unilateral ureteral obstruction rats with contrast-enhanced ultrasounography

YANG Qin; YAO Xicun; SUN Haoran; FU Shengwei; ZHENG Cuimin

doi:10.3874/j.issn.1674-1897.2014.03.L0301

Abstract：Objective To explore renal blood perfusion characteristics in unilateral ureteral obstruction (UUO) rat with contrast-enhanced ultrasound (CEUS), to provide a theoretical basis for clinical noninvasive quantitative evaluation of obstructive nephropathy. Methods Twenty Sprague Dawley rats were used in the study. CEUS were preformed before and 7 days after the UUO operation, and followed with histopathologic examination. Regional perfusion values of renal parenchyma were measured with QLAB software. SPSS16.0 statistical software was used for analasysing blood perfusion time to peak (TTP), slope rate of ascending curve (A), peak intensity (PI),and area under ROC curve (AUC). Results The perfusion values of hydronephrosis renal cortex were significantly changed in UUO rats compared to the preoperative values:TTP (27.42±6.17 s vs. 21.27±4.08s, P<0.05), A (0.88±0.79 dB/s vs. 1.81±0.45 dB/s, P<0.05) and PI (13.60±2.64 dB vs. 16.50±1.36 dB, P<0.05), AUC (1 508.43±481.56 dB·s vs. 1 309.40±306.93) dB·s, P<0.05). The perfusion curve of the contralateral renal cortex was similar to the preoperative within the first 30s, however after 30s, the curve was slowed down significantly. The AUCs (1311.50 ±406.14 dB·s vs. 1209.94 ±298.41 dB·s, P<0.05) were significantly increased. However, A, PI, and TTP had no significant difference between pre-operation and post-operation (P>0.05). Conclusion CEUS and quantitative analysis techniques could reflect the change of kidney perfusion in UUO rats , it provides a theoretical basis for clinical noninvasive quantitative evaluation of obstructive nephropathnephropathy.