Research Progress on DNA Methylation of Gametes,Embryos and Offspring from Assisted Reproductive Technology
HUANG Ru
JIN Meng-han
ZHONG Yu-nong
WANG Ke-han
MENG Qing-xia
Abstract：Assisted reproductive technology(ART)may increase pregnancy risks such as preterm birth and placental dysfunction by perturbing DNA methylation regulation.Studies have shown that the hormonal stimulation and culture conditions during in vitro fertilization(IVF)may induce imprinted gene demethylation;and that the micromanipulation during intracytoplasmic sperm injection(ICSI)may precipitate paternal epigenetic defects.The cryopreservation-thawing stress associated with frozen-thawed embryo transfer(FET)may modify embryonic methylomes;and the ovarian tissue cryopreservation(OTC)ischemia-reperfusion injury may destabilize ovarian DNA methylation.This review delineates the differential mechanisms of these four modalities,including IVF,ICSI,FET and OTC,in regulating DNA methylation of gametes,embryos,and offspring,which may provide a theoretical framework for optimizing ART protocols and improving offspring health.
Keywords：Reproductive techniquesassistedFertilization in vitroSperm injectionsintracytoplasmicEmbryo transferCryopreservationEpigenomicsDNA methylation
Publication Date：2026-01-15
Online Publishing Date：2026-03-19(First online date of this platform, not the publication date of the document)
Pages：4( 45-48 )