Associations between Phthalates Exposure and Inflammatory Cytokines in Ovarian Microenvironment
XIAO Nan
LI Yong-cheng
YAO Yi-ming
SUN Hong-wen
YAO Ru-qiang
CHEN Yong-jun
YIN Yu-chen
LUO Hai-ning
Abstract：Objective:To assess the associations between phthalates(PAEs)exposure and the levels of inflammatory cytokines in the ovarian microenvironment.Methods:64 females who were undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer(IVF/ICSI-ET)from April to December in 2020 were included in this cross-sectional study.The levels of 10 kinds of phthalate metabolites(mPAEs)and 2 kinds factors in follicular fluid were measured.Multivariable linear regression model and further stratified analysis by age were conducted to analyze the associations between mPAEs and inflammatory factors.Results:There were significant positive associations between mono-butyl phthalate(MBP)and tumor necrosis factor-α(8=0.446,P=0.003)and interleukin-6(β=0.425,P=0.003)in the follicular fluid.Similarly,there was a significant positive relationship between mono(2-ethyl-5-oxohexyl)phthalate(MEOHP)and tumor necrosis factor-α(β=0.411,P=0.019).In the stratified analysis,MBP still showed significantly the positive association with tumor necrosis factor-α(β=0.667,P=0.000)and interleukin-6(β=0.407,P=0.028)in the group aged ≥ 30 years.In contrast,there were no correlations between mPAEs and inflammatory factors within the group aged＜30 years.Conclusions:Our results suggest that there is strongly association between PAEs exposure and the altered levels of inflammatory factor,and that PAEs exposure may bring changes of inflammatory burden in the ovarian microenvironment.
Keywords：Phthalic anhydridesFormic acid estersEnvironmental pollutantsFollicular fluidTumor necrosis factor-alphaInterleukin-6Fertilization in vitroSperm injectionsintracytoplasmic
Publication Date：2024-09-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 353-360 )