Cardiovascular Protection of 17β-estradiol by Down-regulating the Expression of MCP-1 and RhoA
LIU Wen-hua
ZHANG Zhi-fen
TANG Shan-shan
CHEN Lin-jie
HUANG Zhe-ren
WEI Shuang-shuang
Abstract：Objective:To observe the regulation of estrogen on the expressions of Monocyte chemotactic factor 1 (MCP-1) and Ras homolog gene family member A (RhoA) in female ovariectomized Sprague-Dawley rats,so as to study the protective effect of estrogen on cardiovascular system.Methods:Forty five female rats aged 12 weeks were randomly divided into five groups:the blank control group (CON,n=10),the ovariectomized group (OVX,n=12),the estrogen-treated group with 17β-estradiol (OVX+E2,n=13) and the sham-operated group (SHAM,n=10).200 μg/(kg·d) 17β-estradiol was administered in the OVX+ E2 group for 16 weeks,the other three groups were given equal normal saline.After 16 weeks,the levels of serum estradiol (E2),follicle-stimulating hormone (FSH),monocyte chemotactic factor 1 (MCP-1) and RhoA were measured by ELISA;the heart tissue was harvested and weighted,the morphological change was observed by HE staining.The levels of tissue silence regulating protein 1 (SIRT1),activated protein kinase (AMPK) were measured by ELISA.The expressions of MCP-1 and RhoA were detected by immunofluorescence histochemistry.Results:The levels of serum and heart tissue RhoA and MCP-1 in the OVX+E2 group were significantly lower than those in the OVX group (P＜0.05).The levels of heart tissue SIRT1 and AMPK in the OVX+E2 group were significantly higher than those in the OVX group (P＜0.05).Conclusions:The estrogen replacement plays a protective effect in those female ovariectomized rats by down-regulating the expressions of serum and heart tissue MCP-1 and RhoA.The potential mechanism is that the AMPK/SIRT1 pathway may be up-regulated by 17β-estradiol.
Keywords：EstradiolSilence regulating protein 1AMP-activated protein kinaseMonocyte chemotactic factor 1Ras homolog gene familymember AAnimal experimentation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 373-377 )