Effects of Estrogen on Osteoporosis in Ovariectomized Rats
WEI Shuang-shuang
ZHANG Zhi-fen
HUANG Zhe-ren
HE Yi-ran
WU Hong-yan
LIU Wen-hua
TANG Shan-shan
HUANG Jian
Abstract：Objective:To investigate the protective effects of exogenous estrogen on bone tissue in a postmenopausal osteoporosis (PMO) rat mode,as well as the effect on the expression of NFATc1.Methods:The 12 weeks Wistar rats were randomly divided into sham operation group,OVX group,and OVX+E2 group and 10 rats in each group.In the OVX+E2 group,17β-F2 was injected subcutaneously and the other two groups were given the same volume of normal saline.After 16 weeks,the serum E2 level was determined by ELISA,the expression of NFATc1 in bone tissue were measured by RT-PCR,the bone mineral density (BMD),the fraction of bone tissues (BV/TV),the thickness of the trabecular bone (Tb.Th) and number of trabecular bone (Tb.N) were measured using Micro-CT analysis.Results:The OVX+E2 group and sham operation group compared with the OVX group,the levels of BMD,BV/TV,Tb.Th,Tb.N and serum E2 were significantly increased,the level of NFATc1 were significantly decreased (P＜0.05).Conclusions:Estrogen may prevent bone loss by decreasing NFATc1 expression in OVX rats.
Keywords：EstrogensOsteoporosispostmenopausalAnimalslaboratoryNuclear factor of activated T-cells cytoplasmic 1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 93-96 )