Toxic Effect of Formaldehyde on Human First Trimester Cytotrophoblasts
CAI Xiao-hui
LIN Qing
Abstract：Objective:To investigate the toxic effect of formaldehyde on the cellular viability and secretory function of human cytotrophoblasts at the first trimester stage. Methods:Human villi were obtained from nine patients undergoing abortion with the signed consent form. Cytotrophoblasts were extracted and cultured in vitro in medium, and then treated with formaldehyde (50,100,150,200 μmol/L) for 48 hours. The cellular viability of cytotrophoblasts was determined with Cell Counting Kit (CCK-8), while the level of human chorionic gonadotropin (hCG) in medium was assayed with radioimmunoassay. DMEM/F12 medium was used as the negative control. Results:The cellular viabilities of cytotrophoblasts in three treatment groups with ≥100μmol/L of formaldehyde was significantly decreased than that in the control group (P<0.05). The differences of the hCG levels among those groups were not significant (P>0.05). Conclusions:Formaldehyde at a higher dose (≥100μmol/L) may reduce the cellular viability of cytotrophoblasts.
Keywords：FormaldehydeChorionic villiChorionic gonadotropinCytotoxicityimmunologic
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 51-52 )