Identification of Aneuploidy with Fluorescence C-banding Staining in Mouse Oocytes
ZHANG Jin-wen
DU Chun-hai
Abstract：Objective:To investigate the oocyte aneuploid in mice with the method of chromosome C-banding staining. Methods:The standard Giemsa C-banding staining and the modified Giemsa C-banding staining were used to show mouse chromosomes, respectively. The DAPI (4′, 6-diamidino-2-phenylindole) staining, so-called fluorescence C-banding staining, was used to show oocytes. Subsequently, the morphology and number of chromosomes were analyzed with fluorescence microscope. Results:Chromosomes were weakly stained by the standard Giemsa C-banding staining, while chromosomes were easily dissolved by the modified Giemsa C-banding staining. The fluorescence C-banding staining showed superiorities, such as the increased contrast degree between the brilliant white centromeres and the strong blue arms of chromosomes, and the shorten dyeing time. Conclusions:The study suggested that the fluorescence C-banding staining was a simple and rapid method to identify aneuploidy of mouse oocytes.
Keywords：MiceOocytesChromosomesAneuploidy
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 271-273 )