Clinical Application of the Next-Generation Sequencing Technology
GAO Chao
SHEN Jian-dong
CUI Yu-gui
Abstract：In recent years, the sequencing technology has been innovated following the breakthrough of the engineering material bottleneck. The high-throughput gene-sequencing technology matured gradually made the transformation from routine and basic research laboratory to clinical practice, because the next-generation sequencing (NGS) technology met the needs of individual diagnosis and treatment. In this paper, the developing progress of NGS technology, and operational process, were introduced. Those mainstream products in the international market at present were also introduced. The Ion Torrent sequencing platform of Life Tech Company was introduced in detail, including its basic principle, laboratory protocol, coefficient of performance and clinical application. This paper also provided some examples to show the specific significance in clinical application and potential insufficient of NGS technology.
Keywords：Sequence analysisMolecular diagnostic techniquesGenetic techniques
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 154-156,171 )