The Association Study of HLA-G Single Nucleotide Polymorphisms and Severe Pre-eclampsia
JIA Yan-ju
CHEN Xu
CUI Hong-yan
CHENG Lan
ZHANG Chun-hong
LI Yan-jie
Abstract：Objective: To detect the distribution of HLA-G SNPs which is one of the susceptibility genes of pre-eclampsia in severe pre-eclampsia patients and normal pregnant women, to understand the change of HLA-G SNPs in the patients with severe pre-eclampsia. Methods:The blood samples were collected from 67 cases of severe pre-eclampsia group and 37 cases of control group, the genomic DNA of samples were extracted, the Exon-2 and Exon-3 of HLA-G SNPs were chosen to evaluate through sequencing, the fluorescent primers were synthesized and PCR was proceeded, the fragments obtained from PCR were sequenced and analyzed to find SNPs. The correlation of SNPs and severe pre-eclampsia was analyzed by statistical analysis. Results:Two SNPs, C374T and G476A were found in the Exon 2 of HLA-G. Three SNPs, C810T, C859A and G954A were found in Exon 3. There was no significant difference in the distribution of the SNPs between pre-eclampsia and normal pregnancies. Conclusions:①Five SNPs were found in the HLA-G Exon 2 and 3, which were not related with severe pre-eclampsia, these SNPs were synonymous mutations.②C374T and G954A were newly discovered HLA-G SNPs which were submitted to the dbSNP and obtained NCBI Assay Id (ss#) 119994701 and 119994702, and the Reference SNP Id (rs#):rs77474325 and rs75783850.
Keywords：Pre-eclampsiaHLA antigenGenesExonsPolymorphismsingle nucleotide
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 80-83 )