Investigation of Anti-Tumor Effect of Taxotere on Nude Mice Xenografts of Human Ovarian Cancer SKOV3 Cells and Its ;Synergistic Effect with Cisplatin,Herceptin

WU Ya-wei; HU Jian-ming; SHI Yi-zhen; YANG Ning

doi:10.3969/j.issn.1674-1870.2017.01.011

Abstract：Objective: To study the anti-tumor effect of Taxotere on nude mice xenografts of human ovarian cancer SKOV3 cells and to compare the anti-tumor effect of its combination with DDP and(or) Herceptin. Methods:An animal model of inoculating SKOV3 cell suspension on the right axillary subcutaneous of nude mice was established, and then the mice were randomized into 8 groups: DDP group, Herceptin group, Taxotere group, DDP+Herceptin group, DDP+Taxotere group, Herceptin+Taxotere group, DDP+Herceptin+Taxotere group and control group, each group included 4 mice. The mice were administrated respectively with DDP (3 mg/kg), Herceptin (20 mg/kg), Taxotere (5 mg/kg) via caudal vein injection once a week for consecutive 6 weeks. The size of the tumor and mice weight were measured weekly, then the mice were put to death in the first week after finishing the whole treatment. The inhibition ratio of tumor growth was calculated and tumor tissues were observed by HE staining. The apoptotic index was analyzed by TUNEL technique. The Ki-67 expression in xenograft tumors were analyzed by immunohistochemical staining. Results:①Taxotere can significantly inhibit the growth of xenografts of human ovarian cancer SKOV3 cells in nude mice, and the inhibition effect is more prominent when it combined with DDP or Herceptin, while the inhibition effect is the most obvious in DDP+Herceptin+Taxotere group;②The tumor cell apoptotic index of Taxotere group was significantly higher than control group, and the apoptotic index is higher when Taxotere was joint with DDP or Herceptin respectively, while the apoptotic index is the highest in DDP+Herceptin+Taxotere group;③The Ki-67 expression ratio in xenograft tumors of Taxotere group was significantly reduced than control group, and Ki-67 expression ratio in xenograft tumors is lower when Taxotere was joint with DDP or Herceptin respectively, while the Ki-67 expression ratio in xenograft tumors is the lowest in DDP+Herceptin+Taxotere group. Conclusions:①Taxotere can obviously inhibit the growth of xenografts of human ovarian cancer SKOV3 cells in nude mice;Down regulation of Ki-67 expression in SKOV3 cells might be one of its possible mechanisms to induce the cell apoptosis; ②There exists synergistic effect of Taxotere, DDP and Herceptin while combining the three drugs can more effectively inhibit the growth of tumor.