EGCG Induce the Apoptosis of Ovarian Cancer SKOV3 Cells and Its Mechanism

ZHOU Fei-fei; DONG Tian-rui; WANG Jing; LIU Yan-qiu; NI Jin-hong

Abstract：Objective:To investigate the effect and mechanism of EGCG on human ovarian cancer SKOV3 cells′apoptosis. Methods:SKOV3 cells were divided into four groups. Negative control group:cultured with normal culture medium;EGCG low dose group:cultured with 1μmol/L EGCG;EGCG middle dose group:cultured with 10μmol/L EGCG;EGCG high dose group:cultured with 100μmol/L EGCG. Cell proliferation inhibition rate of each group of SKOV3 cells were test by MTT assay, nuclei apoptosis were checked by Hoechs staine, cells morphology were investigated by electron microscope, Caspase-3 expression was detected by Western-blot. Results:Cell proliferation inhibition test results showed that the inhibition rate of EGCG low, medium and high concentration group on SKOV3 cells was (81.33±3.71)%, (67.00±7.21)%and (54.67±3.84)%, the difference was statistically significant (P<0.05) compared with the negative control group (101.00 ±3.06)%. Hoechst33258 staining results showed that, apoptosis cell percentage of EGCG low, medium and high concentration group was (16.77 ±2.48)%, (24.33±3.70)%and (39.67±4.11)%, the difference was statistically significant (P<0.05) compared with the negative control group [(2.00±1.45)%]. Electron microscopy showed that with the concentration of EGCG elevated, the damage on ultrastructure of SKOV3 cell became clear. In addition, EGCG could raised Caspase-3 expression levels. Conclusions:EGCG induced ovarian cancer SKOV3 cells apoptosis by enhancing the expression of Caspase-3 gene.