The Protection Effect and Mechanism of Schisandrin B on Extravillous Trophoblast Cell Line Damage Caused by BaP

WU Nian; CHEN Ya-qiong; CHEN Xiao; HOU Hai-yan; WANG Hua

Abstract：Objective:To explore the protection mechanism of Schisandrin B on extravillous trophoblast cell line damage caused by BaP. Methods:After 0.5,1,2μmol/L Sch B deal with human HTR8-SVneo cells 24 h,added 20μmol/L Bap 24 h, using MTS measured cells growth. Used JC-1 fluorescent staining to measure the change of mitochondrial membrane potential in each group. Respectively used ELISA to measure the concentration of Bax and cyt-c protein in each group. Respectively used Western blotting to analyse the expression of caspase-9 in each group. Results: ①MTS results showed that BaP alone cell OD was 1.187±0.015,0.5,1,2μmol/L Sch B the role of OD was 1.483±0.022,1.489±0.048 and 1.531±0.240,compared with BaP alone group,the differences were statistically significant (P<0.05).②JC-1 fluorescent staining results showed that BaP alone cell mitochondrial fluorescent intensity was lower and 0.5,1,2μmol/L Sch B the role of mitochondrial fluorescent intensity was higer. Compared with BaP alone group,the differences were statistically significant (P<0.05).③ELISA results showed that BaP alone cell the concentration of Bax,cyt-c protein were higher and 0.5,1,2μmol/L Sch B the role of the concentration of Bax,cyt-c protein were lower,respectively. Compared with BaP alone group,the differences were statistically significant (P<0.05). ④Western blotting results showed that BaP alone cell the concentration of caspase-9 protein was higer ,and 0.5,1,2μmol/L Sch B the role of the concentration of caspase-9 protein was lower,respectively. Compared with BaP alone group,the differences were statistically significant (P<0.05). Conclusions:Schisandrin B can prevent damage of extravillous trophoblast cell line caused by BaP,whose mechanism may be associated with resistance to mitochondrial pathway.