The Effect of miR-200c Over Expression on OVCAR-3 Epithelial Ovarian Cancer Biological Functions
WU Hai-tian
ZHANG Yue
LIN Qi-de
ZHANG Shu
Abstract：Objective: To investigate the effect of the miR-200c over-expression on the ability of proliferation, migration and invasion in OVCAR-3 ovarian cancer cells. Methods:Constructed retrovirus expression vector pMSCVpuro-miR-200c and transfected OVCAR-3 cells to overexpress miR-200c. By using CCK8 and Transwell methods to detect the ability of OVCAR-3 cells in proliferation and metastasis. Different expression genes involved in OVCAR-3 cells were detected by using gene chip before and after transfected with pMSCVpuro-miR-200c. Results:Compared with untransfected cells, the ability of proliferation, migration and invasion in pMSCVpuro-miR-200c transfected OVCAR-3 cell are significantly decreased (P<0.01). 190 up-regulated genes and 166 down-regulated genes were detected in OVCAR-3 cells after over-expressed miR-200c. Conclusions:As a tumor suppression gene,overexpression of miR-200c could inhibit the proliferation,migration and invasion of ovarian cancer cells.
Keywords：Ovarian neoplasmsCell proliferationCell movementNeoplasm invasivenessmiR-200c
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 509-512 )